![]() This study adds further knowledge on the structure of the LPL gene and its expression in river buffalo, with potential effects on milk qualitative and quantitative production. Haploinsufficiency is proposed as a regulation mechanism. The dual-color electrophoretic mobility shift assay demonstrated that only genotype g.-446GG allowed the binding of the Sp1 transcription factor, resulting in overexpression of the gene (~2.5 fold), as confirmed by the quantitative PCR results. A strong linkage disequilibrium (D′ = 1 r 2 = 0.903) was found between the 2 SNP. Location: Newport News, VA 23604 Race Date: 3 12:00:00 AM (Sunday) Categories: Color Me Rad - Newport News Color Me Rad - Newport News Reviews 5 - 1 reviews Review Color Me Rad - Newport News 5.0/5 Course: 5.0/5 Organization: 3.0/5 RACER I LOVED THE LOCATION AND THE RACE IN GENERAL. We developed PCR-RFLP methods for genotyping the 2 SNP and calculated the allele frequencies. ![]() Eleven SNP were detected, and the attention was focused on the SNP with potential functional effects: g.-446A>G, because the presence of G created a consensus motif for the transcription factor Sp1, and g.107A>G, which was the only exonic SNP. Thirteen consensus sequences for transcription factors were found in the promoter. The aim of this work was to explore the genetic diversity of LPL and its promoter and to identify functional mutations, using a combined approach based on sequencing, dual-color electrophoretic mobility shift assay, and quantitative PCR. SC / NC / VA / SC / OH / WV / ME / MANCHESTER, NH. ![]() The LPL gene has been seldom investigated in dairy ruminants and barely studied in river buffalo ( Bubalus bubalis). You do not have to be fit to do this race I ran this in Boise with my daughter and a big group of friends and had a BLAST Now that I am feeling more healthy and fit I was excited to get in on the fun this year, but you do not have to be fit to run this race. CA / CA / Valhalla, NY / Hybrid Remote / Parole, MD / Pierre, SD / LIVERPOOL, NY / BUFFALO. Lipoprotein lipase (LPL) is a key enzyme for lipid metabolism, playing a fundamental role in the composition of fat in adipose tissue and milk.
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